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Separation of cationic analytes by nonionic micellar electrokinetic chromatography using polyoxyethylene lauryl ether surfactants with different polyoxyethylene length
journal contribution
posted on 2023-05-18, 07:29 authored by Joselito Quirino, Kato, MAlthough nonionic micellar electrokinetic chromatography is used for the separation of charged compounds that are not easily separated by capillary zone electrophoresis, the effect of the hydrophilic moiety of the nonionic surfactant has not been studied well. In this study, the separation of ultraviolet-absorbing amino acids was studied in electrokinetic chromatography using neutral polyoxyethylene lauryl ether surfactants (Adekatol) in the separation solution. The effect of the polyethylene moiety (the number of repeating units was from 6.5 to 50) of the hydrophobic test amino acids (methionine, tryptophan, and tysorine) was studied using a 10 cm effective length capillary. The separation mechanism was based on hydrophobic as well as hydrogen bonding interactions at the micellar surface, which was made of the polyoxyethylene moiety. The length of the polyoxyethylene moiety of the surfactants was not important in nonionic micellar electrokinetic chromatography mode.
History
Publication title
Journal of Separation ScienceVolume
37Issue
18Pagination
2613-2617ISSN
1615-9306Department/School
School of Natural SciencesPublisher
Wiley-V C H Verlag GmbhPlace of publication
1615-9306Rights statement
Copyright 2014 Wiley-VCH VerlagRepository Status
- Restricted