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The floral transcriptome of Eucalyptus grandis

journal contribution
posted on 2023-05-18, 06:15 authored by Vining, KJ, Romanel, E, Rebecca JonesRebecca Jones, Klocko, A, Alves-Ferreira, M, Hefer, CA, Amarasinghe, V, Dharmawardhana, P, Naithani, S, Ranik, M, Wesley-Smith, J, Solomon, L, Jaiswal, P, Myburg, AA, Strauss, SH

  • As a step toward functional annotation of genes required for floral initiation and development within the Eucalyptus genome, we used short read sequencing to analyze transcriptomes of floral buds from early and late developmental stages, and compared these with transcriptomes of diverse vegetative tissues, including leaves, roots, and stems.

  • A subset of 4807 genes (13% of protein-coding genes) were differentially expressed between floral buds of either stage and vegetative tissues. A similar proportion of genes were differentially expressed among all tissues. A total of 479 genes were differentially expressed between early and late stages of floral development. Gene function enrichment identified 158 gene ontology classes that were overrepresented in floral tissues, including ‘pollen development’ and ‘aromatic compound biosynthetic process’. At least 40 floral-dominant genes lacked functional annotations and thus may be novel floral transcripts.

  • We analyzed several genes and gene families in depth, including 49 putative biomarkers of floral development, the MADS-box transcription factors, ‘S-domain’-receptor-like kinases, and selected gene family members with phosphatidylethanolamine-binding protein domains. Expanded MADS-box gene subfamilies in Eucalyptus grandis included SUPPRESSOR OF OVEREXPRESSION OF CO 1 (SOC1), SEPALLATA (SEP) and SHORT VEGETATIVE PHASE (SVP) Arabidopsis thaliana homologs.

  • These data provide a rich resource for functional and evolutionary analysis of genes controlling eucalypt floral development, and new tools for breeding and biotechnology.

    • History

    Publication title

    New Phytologist

    Volume

    206

    Issue

    4

    Pagination

    1406-1422

    ISSN

    0028-646X

    Department/School

    School of Natural Sciences

    Publisher

    Blackwell Publishing Ltd

    Place of publication

    9600 Garsington Rd, Oxford, England, Oxon, Ox4 2Dg

    Rights statement

    Copyright 2014 New Phytologist

    Repository Status

    • Restricted

    Socio-economic Objectives

    Hardwood plantations

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      University Of Tasmania

      Categories

      Keywords

      Eucalyptusfloral transcriptomefloweringMADS-box

      Licence

      In Copyright

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