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Development and application of molecular methods (PCR) for detection of Tasmanian Atlantic salmon reovirus

journal contribution
posted on 2023-05-18, 05:24 authored by Zainathan, SC, Carlile, G, Carson, J, McColl, KA, Crane, MStJ, Williams, LM, Hoad, J, Moody, NJG, Aiken, HM, Browning, GF, Barbara NowakBarbara Nowak
Molecular (PCR) diagnostic tests for the detection and identification of aquareovirus in general, and Tasmanian Atlantic salmon reovirus (TSRV) specifically, were developed, and their diagnostic sensitivity and specificity were determined and compared with virus isolation in cell culture. Intralaboratory and interlaboratory comparison of PCR (conventional hemi-nested RT-PCR & RTqPCR) and virus isolation in cell culture using finfish cell lines, CHSE-214 and EPC, was carried out for the detection and identification of TSRV using field samples of farmed Atlantic salmon Salmo salar, L. from various aquaculture sites around Tasmania. The interlaboratory comparison of diagnostic methods was carried out between two laboratories, AAHL-CSIRO and DPIPWE Tasmania. A total of 144 fish from nine sites (12–33 fish per site) were sampled from two regions of Tasmania (Tamar River estuary in the north and Huon River estuary in the south-east) during late spring to early summer of 2009, and the data were analysed using different statistical approaches. The prevalence of TSRV ranged from 6% to 22% in both regions. All the diagnostic methods (data from both laboratories) had high specificity, while the estimated sensitivity varied between tests with RT-qPCR being the most sensitive (95.2%) method followed by virus isolation and then conventional hemi-nested RT-PCR.

History

Publication title

Journal of Fish Diseases

Volume

38

Issue

8

Pagination

739-754

ISSN

0140-7775

Department/School

Institute for Marine and Antarctic Studies

Publisher

Blackwell Publishing Ltd

Place of publication

9600 Garsington Rd, Oxford, England, Oxon, Ox4 2Dg

Rights statement

Copyright 2014 John Wiley & Sons Ltd

Repository Status

  • Restricted

Socio-economic Objectives

Aquaculture fin fish (excl. tuna)

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