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Respirometry: Correcting for diffusion and validating the use of plastic multiwell plates with integrated optodes

journal contribution
posted on 2023-05-17, 23:19 authored by Elias Polymeropoulos, Elliott, NG, Wotherspoon, SJ, Peter FrappellPeter Frappell
Polystyrene multiwell plates with integrated optodes act as multiple closed-system respirometers that enable the simultaneous measurement of oxygen consumption in small animals. However, the diffusion of oxygen through polystyrene needs to be taken into consideration. Here we provide an equation that accounts for the empirically determined rate of oxygen through a polystyrene well when calculating the instantaneous rate of oxygen consumption. Furthermore, we describe a novel method of calibrating a small respirometer for accuracy using micro-osmotic pumps containing an oxygen scavenger, which is delivered at a constant rate and therefore yields a constant rate of oxygen consumption in an airtight system.

History

Publication title

Physiological and Biochemical Zoology

Volume

86

Issue

5

Pagination

588-592

ISSN

1522-2152

Department/School

School of Natural Sciences

Publisher

University of Chicago Press

Place of publication

Chicago, USA

Rights statement

Copyright 2013 University of Chicago

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the physical sciences

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