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Ion flux measurements using the MIFE technique


Shabala, S and Shabala, L and Bose, J and Cuin, T and Newman, I, Ion flux measurements using the MIFE technique, Plant Mineral Nutrients: Methods and Protocols, Humana Press, FJM Maathuis (ed), United States of America, pp. 171-183. ISBN 978-1-62703-151-6 (2013) [Other Book Chapter]

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Copyright 2013 Springer Science+ Business Media, LLC (Information transferred from record 84137, which was the same paper but which was originally entered as an A1, and which has been deleted. HW 28/2/14)

DOI: doi:10.1007/978-1-62703-152-3_11


Noninvasive microelectrode ion flux measurements (the MIFE™ technique) allow the concurrent quantification of net fluxes of several ions with high spatial (several μm) and temporal (ca 5 s) resolution. The MIFE technique has become a popular tool for studying the adaptive responses of plant cells and tissues to a large number of abiotic and biotic stresses. This chapter briefly summarizes some key findings on spatial and temporal organization of plant nutrient acquisition obtained by the MIFE technique, as well as the MIFE contribution towards elucidating the mechanisms behind a plant's perception and signaling of major abiotic stresses. The full protocols for microelectrode fabrication, calibration, and use are then given, and two basic routines for mapping root ion flux profiles and studying transient ion flux kinetics are given.

Item Details

Item Type:Other Book Chapter
Keywords:ion flux, membrane transport, ion-selective microelectrodes, deficiency, toxicity, acidity, salinity, oxidative stress
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Environmentally sustainable plant production
Objective Field:Environmentally sustainable plant production not elsewhere classified
UTAS Author:Shabala, S (Professor Sergey Shabala)
UTAS Author:Shabala, L (Associate Professor Lana Shabala)
UTAS Author:Bose, J (Dr Jayakumar Bose)
UTAS Author:Cuin, T (Dr Tracey Cuin)
UTAS Author:Newman, I (Dr Ian Newman)
ID Code:89286
Year Published:2013
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2014-02-27
Last Modified:2014-06-13

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