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Ion-exclusion chromatography of aliphatic carboxylic acids on an unmodified silica gel column


Ohta, K and Tanaka, K and Haddad, PR, Ion-exclusion chromatography of aliphatic carboxylic acids on an unmodified silica gel column, Journal of Chromatography A, 739, (1-2) pp. 359-365. ISSN 0021-9673 (1996) [Refereed Article]

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DOI: doi:10.1016/0021-9673(96)00213-0


In order to demonstrate the effectiveness of a silica gel column in ion-exclusion chromatography, the elution behavior of aliphatic carboxylic acids was investigated on an unmodified silica gel column having cation-exchange characteristics. When water was used as an eluent, the elution order of the carboxylic acids was dependent on the pKa and the hydrophobicity, but the resolution between strong acids, e.g. HNO3, and carboxylic acids was not satisfactory. Therefore, the composition of eluents containing sulfuric acid and organic modifiers was investigated for the separation of HNO3 and C1C10 aliphatic carboxylic acids. When 0.5 mM sulfuric acid containing 0.05% heptanol was used as an eluent, an excellent ion-exclusion chromatographic separation was achieved in 30 min using UV-detection at 220 nm.

Item Details

Item Type:Refereed Article
Keywords:Mobile phase composition; Organic modifiers; Carboxylic acids; Sulfuric acid
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Instrumental methods (excl. immunological and bioassay methods)
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:8700
Year Published:1996
Web of Science® Times Cited:43
Deposited By:Chemistry
Deposited On:1996-08-01
Last Modified:2010-03-11

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