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Effect of immunization route on mucosal and systemic immune response in Atlantic salmon (Salmo salar)

journal contribution
posted on 2023-05-17, 19:26 authored by Valdenegro-Vega, VA, Crosbie, P, Vincent, B, Cain, KD, Barbara NowakBarbara Nowak
This study aimed to assess systemic and mucosal immune responses of Atlantic salmon (Salmo salar) exposed to two protein-hapten antigens – dinitrophenol (DNP) and fluorescein isothiocyanate (FITC) each conjugated with keyhole limpet haemocyanin (KLH) – administered using different delivery strategies. Fish were exposed to the antigens through different routes, and were given a booster 4 weeks post initial exposure. Both systemic and mucosal antibody responses were measured for a period of 12 weeks using an enzyme-linked immunosorbent assay (ELISA). Only fish exposed to both antigens via intraperitoneal (IP) injection showed increased systemic antibody response starting 6 weeks post immunization. No treatment was able to produce a mucosal antibody response; however there was an increase in antibody levels in the tissue supernatant from skin explants obtained 12 weeks post immunization from fish injected with FITC. Western blots probed with serum and culture supernatant from skin explants showed a specific response against the antigens. In conclusion, IP injection of hapten-antigen in Atlantic salmon was the best delivery route for inducing an antibody response against these antigens in this species. Even though IP injection did not induce an increase in antibody levels in the skin mucus, there was an increased systemic antibody response and an apparent increase of antibody production in mucosal tissues as demonstrated by the increased level of specific antibody levels in supernatants from the tissue explants.

History

Publication title

Veterinary Immunology and Immunopathology

Volume

151

Issue

1-2

Pagination

113-123

ISSN

0165-2427

Department/School

Institute for Marine and Antarctic Studies

Publisher

Elsevier Science Bv

Place of publication

Po Box 211, Amsterdam, Netherlands, 1000 Ae

Rights statement

Copyright 2013 Elsevier B.V.

Repository Status

  • Restricted

Socio-economic Objectives

Aquaculture fin fish (excl. tuna)

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