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Effect of immunization route on mucosal and systemic immune response in Atlantic salmon (Salmo salar)

Citation

Valdenegro-Vega, VA and Crosbie, P and Vincent, B and Cain, KD and Nowak, BF, Effect of immunization route on mucosal and systemic immune response in Atlantic salmon (Salmo salar), Veterinary Immunology and Immunopathology, 151, (1-2) pp. 113-123. ISSN 0165-2427 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 Elsevier B.V.

DOI: doi:10.1016/j.vetimm.2012.10.010

Abstract

This study aimed to assess systemic and mucosal immune responses of Atlantic salmon (Salmo salar) exposed to two protein-hapten antigens dinitrophenol (DNP) and fluorescein isothiocyanate (FITC) each conjugated with keyhole limpet haemocyanin (KLH) administered using different delivery strategies. Fish were exposed to the antigens through different routes, and were given a booster 4 weeks post initial exposure. Both systemic and mucosal antibody responses were measured for a period of 12 weeks using an enzyme-linked immunosorbent assay (ELISA). Only fish exposed to both antigens via intraperitoneal (IP) injection showed increased systemic antibody response starting 6 weeks post immunization. No treatment was able to produce a mucosal antibody response; however there was an increase in antibody levels in the tissue supernatant from skin explants obtained 12 weeks post immunization from fish injected with FITC. Western blots probed with serum and culture supernatant from skin explants showed a specific response against the antigens. In conclusion, IP injection of hapten-antigen in Atlantic salmon was the best delivery route for inducing an antibody response against these antigens in this species. Even though IP injection did not induce an increase in antibody levels in the skin mucus, there was an increased systemic antibody response and an apparent increase of antibody production in mucosal tissues as demonstrated by the increased level of specific antibody levels in supernatants from the tissue explants.

Item Details

Item Type:Refereed Article
Keywords:Atlantic salmon, mucosal immunity, hapten-antigens, immunization
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Fisheries sciences
Research Field:Fish pests and diseases
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Aquaculture fin fish (excl. tuna)
UTAS Author:Valdenegro-Vega, VA (Miss Victoria Valdenegro)
UTAS Author:Crosbie, P (Dr Philip Crosbie)
UTAS Author:Vincent, B (Miss Benita Vincent)
UTAS Author:Cain, KD (Associate Professor ken Cain)
UTAS Author:Nowak, BF (Professor Barbara Nowak)
ID Code:86381
Year Published:2013
Web of Science® Times Cited:27
Deposited By:NC Marine Conservation and Resource Sustainability
Deposited On:2013-09-11
Last Modified:2021-03-19
Downloads:0

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