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Microsatellite primer development for the seagrass Zostera nigricaulis (Zosteraceae)

journal contribution
posted on 2023-05-17, 18:35 authored by Smith, TM, York, PH, Stanley, AM, Macreadie, PI, Keough, MJ, Donald RossDonald Ross, Sherman, CDH
Seagrasses are marine angiosperms with a worldwide distribution that form conspicuous beds in nearshore habitats. Despite being universally recognised as a foundation species that performs a number of important ecosystems functions (incl. sediment stabilisation, facilitation of biodiversity, nutrient cycling and carbon sequestration), global seagrass habitats are in decline. Resilience-the ability to recover from disturbance without switching to an alternative state-is paramount to the maintenance and persistence of seagrass habitats. Genetic diversity is a key component of seagrass resilience and contributes to an understanding of population structure, connectivity between populations, and reproductive strategies. Microsatellite primers were developed to investigate the resilience of the seagrass Zostera nigricaulis, which dominates subtidal habitats in the bays of south-eastern Australia.Wealso tested for cross-amplification of markers between Z. nigricaulis and previously developed markers for the sympatric species Z. muelleri to assess their applicability for use in assessing patterns of genetic diversity, population structure, and mating system. Using next-generation sequencing we isolated 11 novel microsatellite loci for Z. nigricaulis, 8 of which were polymorphic for the samples tested. Allelic diversity ranged from 1 to 8. None of the primer pairs developed for Z. nigricaulis cross-amplified in Z. muelleri; but 14 of 24 primer pairs previously developed for Z. muelleri amplified clearly in Z. nigricaulis samples with six of these showing polymorphism. The results demonstrate the applicability of the Z. nigricaulis microsatellite primers for use in the study of population genetics and limited cross-amplification with Z. muelleri.

History

Publication title

Conservation Genetic Resources

Volume

5

Pagination

607-610

ISSN

1877-7252

Department/School

Institute for Marine and Antarctic Studies

Publisher

Springer Netherlands

Place of publication

Netherlands

Rights statement

Copyright 2013 Springer Science+Business Media Dordrecht

Repository Status

  • Restricted

Socio-economic Objectives

Assessment and management of terrestrial ecosystems

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