eCite Digital Repository

The expression of fetuin in the development and maturation of the hemopoietic and immune systems


Dziegielewska, KM and Brown, WM and Deal, A and Foster, KA and Fry, EJ and Saunders, NR, The expression of fetuin in the development and maturation of the hemopoietic and immune systems, Histochemistry and Cell Biology, 106, (3) pp. 319-330. ISSN 0301-5564 (1996) [Refereed Article]

DOI: doi:10.1007/s004180050048


The distribution and expression of fetuin, a fetal plasma protein that has been shown to have a widespread intracellular presence in many developing tissues including the central nervous system, has been studied in the developing immune and hemopoietic organs of fetal and adult sheep. The presence of fetuin was demonstrated using immuno-cytochemistry and expression of fetuin was studied using northern blot analysis and in situ hybridization. In the developing sheep fetus, fetuin was shown to be expressed first in the hemopoietic cells of the fetal liver and subsequently in the forming spleen. The very first stromal, bone marrow-forming cells, also expressed fetuin mRNA. These cells became more numerous during gestation and by embryonic day (E)115 (term is 150 days), fetuin-expressing cells were identified morphologically to be monocytes/macrophages. Fetuin protein, on the other hand, was present in all hemopoietic and immune organs from the earliest age studied (E30) but was confined initially to matrix, mesenchymal tissue. Fetuin-positive cells could be identified in the spleen at E60 as early hemopoietic cells, in the lymph nodes at E60 as stromal cells and macrophages, and at E115 in the thymus as macrophages and squamous cells. In the adult, fetuin mRNA was only detectable by northern blot in the liver and the bone marrow. Using in situ hybridization in adult tissue, fetuin mRNA-positive cells were identified in the bone marrow to be monocytes/macrophages. Additionally, in the spleen germinal centres, fetuin mRNA was identified in cells with the morphology of dendritic cells. Using three separate cellular markers: lysozyme, S-100, and α1-antitrypsin, the cellular identification of fetuin-positive cells was confirmed to be in the monocyte/macrophage lineage.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Medical biochemistry and metabolomics
Research Field:Medical biochemistry - proteins and peptides (incl. medical proteomics)
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the environmental sciences
UTAS Author:Dziegielewska, KM (Dr Kate Dziegielewska)
UTAS Author:Brown, WM ( Brown)
UTAS Author:Deal, A ( Deal)
UTAS Author:Foster, KA (Ms Kelley Anne Foster)
UTAS Author:Fry, EJ (Ms Elizabeth Jane Fry)
UTAS Author:Saunders, NR (Professor Norman Saunders)
ID Code:8251
Year Published:1996
Web of Science® Times Cited:31
Deposited By:Anatomy and Physiology
Deposited On:1996-08-01
Last Modified:2011-08-19

Repository Staff Only: item control page