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Temperature pulsing for controlling chromatographic resolution in capillary liquid chromatography


Causon, TJ and Cortes, HJ and Shellie, RA and Hilder, EF, Temperature pulsing for controlling chromatographic resolution in capillary liquid chromatography, Analytical Chemistry, 84, (7) pp. 3362-3368. ISSN 0003-2700 (2012) [Refereed Article]

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Copyright Statement

Copyright 2012 American Chemical Society

DOI: doi:10.1021/ac300161b


In this study we introduce the implementation of rapid temperature pulses for selectivity tuning in capillary liquid chromatography. Short temperature pulses improved resolution in discrete sections of chromatograms, demonstrated for ion-exchange chromatography (IC) and hydrophilic interaction chromatography (HILIC) modes. Using a resistively heated column module capable of accurate and rapid temperature changes, this concept is first illustrated with separations of small anions by IC using a packed capillary column as well as a series of nucleobases and nucleosides by HILIC using a silica monolithic column with zwitterionic functionality (ZIC-HILIC). Both positive (increasing temperature) and negative temperature pulses are demonstrated to produce significant changes in selectivity and are useful approaches for improving resolution between coeluted compounds. The approach was shown to be reproducible over a large number of replicates. Finally, the use of temperature gradients as well as other complex temperature profiles was also examined for both IC and HILIC separations.

Item Details

Item Type:Refereed Article
Keywords:chromatographic analysis, lasers, separation, silica, liquid chromatography
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Causon, TJ (Mr Timothy Causon)
UTAS Author:Cortes, HJ (Dr Hernan Cortes)
UTAS Author:Shellie, RA (Associate Professor Robert Shellie)
UTAS Author:Hilder, EF (Professor Emily Hilder)
ID Code:79510
Year Published:2012
Web of Science® Times Cited:17
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2012-09-18
Last Modified:2013-04-22
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