Fernandez Piquer, J and Bowman, JP and Ross, T and Tamplin, ML, Molecular analysis of the bacterial communities in the live Pacific oyster (Crassostrea gigas) and the influence of postharvest temperature on its structure, Journal of Applied Microbiology, 112, (6) pp. 1134-1143. ISSN 1364-5072 (2012) [Refereed Article]
Copyright 2012 The Society for Applied Microbiology
Aims: To evaluate the effect of postharvest temperature on bacterial communities in live Pacific oysters (Crassostrea gigas) using nonculture-based methods.
Methods and Results: Live oysters were compared before and after storage at 4, 6, 15, 20 and 30°C using terminal restriction fragment length polymorphism (T-RFLP). Bacterial communities in freshly harvested (control) vs stored oysters were significantly different. Changes in bacterial communities at 4, 15 and 30°C observed by T-RFLP were further investigated by clone library analysis. Members of the Proteobacteria predominated (43.0–57.0% of clones) in control oysters, while storage altered the bacterial profile. At 4°C, Psychrilyobacter spp.(phylum Fusobacteria) predominated (43.8% of clones), while at 15 and 30°C, members of the phylum Bacteroidetes represented 63.0 and 60.2% of clones, respectively. High microbial diversity in oysters was observed, with at least 73 different genera-related clones among all samples.
Conclusions: Changes in the overall bacterial community of Pacific oysters were influenced by storage temperature and would likely not be detected by standard culture-based methods currently used to assess oyster quality. Certain dominant genera, such as Psychrilyobacter, Polynucleobacter and a bacterial group related to Alkaliflexus, should be further studied as possible indicators for postharvest temperature control.Significance and Impact of the Study: This work is the first report describing the effect of different storage temperatures on bacterial diversity in postharvest live Pacific oysters using molecular-based methods.
|Item Type:||Refereed Article|
|Keywords:||16S rRNA, bacterial community analysis, clone library, Crassostrea gigas, Pacific oyster, T-RFLP|
|Research Division:||Biological Sciences|
|Research Field:||Microbiology not elsewhere classified|
|Objective Group:||Public health (excl. specific population health)|
|Objective Field:||Food safety|
|UTAS Author:||Fernandez Piquer, J (Dr Judith Fernandez-Piquer)|
|UTAS Author:||Bowman, JP (Associate Professor John Bowman)|
|UTAS Author:||Ross, T (Professor Tom Ross)|
|UTAS Author:||Tamplin, ML (Professor Mark Tamplin)|
|Web of Science® Times Cited:||42|
|Deposited By:||Agricultural Science|
|Downloads:||7 View Download Statistics|
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