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Validation and quantification of extractable age pigments for determining the age of Antarctic krill (Euphausia superba)


McGaffin, AF and Nicol, S and Virtue, P and Hirano, Y and Matsuda, T and Uchida, I and Candy, SG and Kawaguchi, S, Validation and quantification of extractable age pigments for determining the age of Antarctic krill (Euphausia superba), Marine Biology: International Journal on Life in Oceans and Coastal Waters, 158, (8) pp. 1743-1755. ISSN 0025-3162 (2011) [Refereed Article]

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Copyright 2011 Springer-Verlag

DOI: doi:10.1007/s00227-011-1688-5


Antarctic krill, Euphausia superba, hatched from eggs and maintained for four years, were sampled periodically for age-pigment analysis. Extractable pigments from the eye and eyestalk ganglia were quantified using fluorescence intensity and standardised against protein. Three peak fluorescence intensities were detected at wavelengths of excitation 280 nm, emission 625 nm (pigment 1); excitation 355 nm, emission 510 nm (pigment 2); and excitation 463 nm, emission 620 nm (pigment 3). There was a positive correlation between the quantity of pigments 1 and 3 and the age of Antarctic krill. A model was developed to predict age from pigment 3 and to compare it with other age proxies (carapace length and eyeball diameter). The quantity of pigment 3 was the best predictor of age. The pigment method can discriminate between similar sized krill aged 12 and 36 months. Age pigments provide an improved tool for age estimation in Antarctic krill, particularly if used in conjunction with other demographic information.

Item Details

Item Type:Refereed Article
Keywords:Antarctic krill, age determination
Research Division:Biological Sciences
Research Group:Ecology
Research Field:Marine and estuarine ecology (incl. marine ichthyology)
Objective Division:Environmental Management
Objective Group:Management of Antarctic and Southern Ocean environments
Objective Field:Biodiversity in Antarctic and Southern Ocean environments
UTAS Author:McGaffin, AF (Ms Angela McGaffin)
UTAS Author:Virtue, P (Associate Professor Patti Virtue)
ID Code:76372
Year Published:2011
Web of Science® Times Cited:4
Deposited By:IMAS Research and Education Centre
Deposited On:2012-03-05
Last Modified:2012-05-24

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