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Selective Vulnerability of Non-Myelinated Axons to Stretch Injury in an In Vitro Co-Culture System


Staal, JA and Vickers, JC, Selective Vulnerability of Non-Myelinated Axons to Stretch Injury in an In Vitro Co-Culture System, Journal of Neurotrauma, 28, (5) pp. 841-847. ISSN 0897-7151 (2011) [Refereed Article]

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Copyright Statement

Copyright 2011 Mary Ann Liebert, Inc.

DOI: doi:10.1089/neu.2010.1658


Diffuse axonal injury (DAI) is an evolving axonopathy commonly characterized clinically as widespread damage to the white matter tracts. In recent electrophysiological studies, researchers have proposed that myelinated and unmyelinated axons differ in their vulnerability and functional recovery following DAI. In this study we present for the first time an in vitro stretch-injury approach that utilizes a novel myelinating co-culture system to determine the differential response between myelinated and non-myelinated axon bundles to injury. In implementing this technique we demonstrate that myelinated axon bundles are less vulnerable to stretch injury compared to caliber-matched non-myelinated bundles. Interestingly, moderate axonal strain did not induce demyelination, but instead caused an increase in the proportion of degenerated myelin basic protein over time. Additionally, there were no significant differences in the expression of axonal swellings, which is indicative of disrupted axonal transport. In summary, we present an ideal in vitro model that permits further mechanistic investigations into the role of myelin and oligodendrocyte-neuron interactions in response to DAI.

Item Details

Item Type:Refereed Article
Keywords:axon myelination; diffuse axonal injury; oligodendrocytes; traumatic brain injury
Research Division:Biomedical and Clinical Sciences
Research Group:Neurosciences
Research Field:Neurology and neuromuscular diseases
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Staal, JA (Dr Jerome Staal)
UTAS Author:Vickers, JC (Professor James Vickers)
ID Code:74972
Year Published:2011
Web of Science® Times Cited:28
Deposited By:Menzies Institute for Medical Research
Deposited On:2011-12-20
Last Modified:2017-11-06
Downloads:1 View Download Statistics

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