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SOCS3 negatively regulates LIF signaling in neural precursor cells


Emery, B and Merson, TD and Snell, C and Young, KM and Ernst, M and Kilpatrick, TJ, SOCS3 negatively regulates LIF signaling in neural precursor cells, Molecular and Cellular Neurosciences, 31, (4) pp. 739-747. ISSN 1044-7431 (2006) [Refereed Article]

DOI: doi:10.1016/j.mcn.2006.01.005


Cytokines that signal through the LIFRβ/gp130 receptor complex, including LIF and CNTF, promote the self-renewal of embryonic and adult neural precursor cells (NPCs). In non-CNS tissues, the protein suppressor of cytokine signaling-3 (SOCS3) negatively regulates signaling through gp130. Here, we analyze the role of SOCS3 in inhibiting LIF signaling in NPCs in vitro. SOCS3 is rapidly expressed by NPCs in response to LIF stimulation, with this expression largely dependent on recruitment of STAT proteins to the activated gp130 receptor. Proliferating NPC cultures can be generated from SOCS3 knockout (SOCS3KO/KO) embryos and display prolonged STAT3 phosphorylation and induction of the GFAP gene in response to LIF. In comparison with SOCS3 wild-type (SOCS3WT/WT) NPCs, SOCS3KO/KO cultures display enhanced self-renewal capacity. However, the clonal potential of SOCS3 WT/WT but not SOCS3KO/KO NPCs is enhanced by exogenous LIF. Thus, SOCS3 acts as a negative regulator of LIF signaling in NPCs. © 2006 Elsevier Inc. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Neurosciences
Research Field:Cellular nervous system
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Young, KM (Associate Professor Kaylene Young)
ID Code:73677
Year Published:2006
Web of Science® Times Cited:22
Deposited By:Research Division
Deposited On:2011-10-21
Last Modified:2011-10-21

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