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Enhanced platelet activating factor receptor (PAFR) expression in smoker airways


Sohal, SS and Mudway, IS and Weston, S and Grigg, J and Walters, H, Enhanced platelet activating factor receptor (PAFR) expression in smoker airways, European Respiratory Journal 38 (Supplement 55), 24-28 September 2011, Amsterdam, The Netherlands, pp. 625s. (2011) [Conference Extract]


Introduction: Cigarette smoking increases the risk of pneumococcal disease, and epidemiologic studies suggest that air pollution from cigarette smoke increases vulnerability to bacterial pneumonia. We have shown that inhalable particulate matter (PM) stimulates pneumococcal adhesion to human lower airway cells. Increased pneumococcal adhesion is associated with increased invasiveness of bacteria. Oxidative stress mediates the adhesive response of airway epithelial cells, and platelet activating factor receptor (PAFR) is a receptor co-opted by pneumococci to facilitate PM-stimulated adhesion. Objective: To investigate the expression of PAFR in the airways of normal lung function smokers (NS) in vivo. Methods: Endobronchial biopsies from 16 NS and 11 normal controls (NC) were stained for anti-PAFR monoclonal antibody. PAFR expression was assessed as percentage of epithelium stained for PAFR over total basement membrane length by using computer-assisted image analysis. Results: Percentage of epithelial staining for PAFR was increased significantly in NS (A) compared to NC (B) (Chi-Square=30.3, 0 (04.8), p<0.001). Conclusions: This is the first description of in-vivo expression of PAFR in the epithelium of NS compared to NC. Our data suggest that enhanced PAFR expression may be the mechanism of increased vulnerability of smokers to pneumococcal infection.

Item Details

Item Type:Conference Extract
Research Division:Biomedical and Clinical Sciences
Research Group:Cardiovascular medicine and haematology
Research Field:Respiratory diseases
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Sohal, SS (Dr Sukhwinder Sohal)
UTAS Author:Weston, S (Mr Steven Weston)
UTAS Author:Walters, H (Professor Haydn Walters)
ID Code:73589
Year Published:2011
Deposited By:Menzies Institute for Medical Research
Deposited On:2011-10-18
Last Modified:2011-10-18

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