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Kinetic performance optimisation for liquid chromatography: Principles and practice
journal contribution
posted on 2023-05-17, 07:33 authored by Causon, TJ, Broekhoven, K, Emily HilderEmily Hilder, Robert ShellieRobert Shellie, Desmet, G, Eeltink, SThis HPLC tutorial focuses on the preparation and use of kinetic plots to characterise the performance in isocratic and gradient LC. This graphical approach allows the selection of columns (i.e. optimum particle size and column length) and LC conditions (operating pressure and temperature) to generate a specific number of plates or peak capacity in the shortest possible analysis time. Instrument aspects including the influence of extracolumn effects (maximum allowable system volume) and thermal operating conditions (oven type) on performance are discussed. In addition, the performance characteristics of porous-shell particle-packed columns and monolithic stationary phases are presented and the potential of future column designs is discussed.
Funding
Australian Research Council
History
Publication title
Journal of Separation ScienceVolume
34Issue
8Pagination
877-887ISSN
1615-9306Department/School
School of Natural SciencesPublisher
Wiley-V C H Verlag GmbhPlace of publication
Weinheim, GermanyRights statement
The definitive published version is available online at: http://onlinelibrary.wiley.com/Repository Status
- Restricted