University of Tasmania
Browse

File(s) not publicly available

The effect of temperature on post-prandial protein synthesis in juvenile barramundi, Lates calcarifer

journal contribution
posted on 2023-05-17, 04:57 authored by Robin Katersky BarnesRobin Katersky Barnes, Christopher CarterChristopher Carter
The experiment aimed to measure post-prandial protein synthesis at three different temperatures. Juvenile barramundi (10.81 ± 3.46 g) were held at 21, 27 and 33 °C and fed to satiation daily. Samples were taken over a 24 h period at 0 (24 h after the previous meal) and then at 4, 8, 12 and 24 h after feeding to measure protein synthesis in the white muscle, liver and remaining carcass. Protein synthesis at 27 and 33 °C peaked 4 h after feeding in all tissues and returned to pre-feeding rates by 12 h. At 21 °C protein synthesis remained constant over 24 h in all tissues. While the concentration of RNA remained stable over the 24 h cycle and across temperatures, the ribosomal activity increased after feeding. This meant kRNA, not the absolute amount of RNA, was the driving force underlying the post-prandial increase in protein synthesis. However, relative differences in protein synthesis between tissues were attributed to differences in RNA concentration. There was a significant positive relationship between white muscle and whole body protein synthesis. This was the first study to show an interaction between temperature and the time after feeding on protein synthesis for an ectotherm, and that a post-prandial peak in protein synthesis only occurred under optimum temperature conditions. © 2010 Elsevier Inc. All rights reserved.

History

Publication title

Comparative Biochemistry and Physiology. Part A: Molecular and Integrative Physiology

Volume

156

Issue

4

Pagination

529-536

ISSN

1095-6433

Department/School

Institute for Marine and Antarctic Studies

Publisher

Elsevier Science Inc

Place of publication

360 Park Ave South, New York, USA, Ny, 10010-1710

Repository Status

  • Restricted

Socio-economic Objectives

Aquaculture fin fish (excl. tuna)

Usage metrics

    University Of Tasmania

    Categories

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC