eCite Digital Repository

Improving controlled pollination methodology for breeding Acacia mangium Willd


Griffin, AR and Vuong, TD and Harbard, JL and Wong, CY and Brooker, C and Vaillancourt, RE, Improving controlled pollination methodology for breeding Acacia mangium Willd, New Forests: Journal of Biology, Biotechnology, and Management of Afforestation and Reforestation, 40, (2) pp. 131-142. ISSN 0169-4286 (2010) [Refereed Article]

DOI: doi:10.1007/s11056-010-9188-x


Acacia mangium is a major plantation species for the pulp and paper industry in south-east Asia and there are a number of active breeding programs. The species is predominantly outcrossing, but with a demonstrated capacity to set selfed seed where outcross pollen is limited, with consequent inbreeding depression in the progeny. Current controlled pollination methods therefore include a time-consuming emasculation step. We used microsatellite genotyping of seedlings to determine the consequences of outcross pollination with and without emasculation. Only 1 of 3 mother trees set a small amount (5%) of selfed seed. Using whole inflorescences from the male parent as the pollen applicator rather than sieved pollen reduced outcross contamination rates from 19.1 to 8.7% and substantially increased worker productivity. Application of sugar solution to the female flowers immediately prior to pollination increased yield of sound seeds per spike. Additional improvements to the pollination protocols are discussed. © 2010 Springer Science+Business Media B.V.

Item Details

Item Type:Refereed Article
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Forestry sciences
Research Field:Tree improvement (incl. selection and breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Forestry
Objective Field:Harvesting and transport of forest products
UTAS Author:Griffin, AR (Professor Rod Griffin)
UTAS Author:Vuong, TD (Mr Duc Tran)
UTAS Author:Harbard, JL (Ms Jane Harbard)
UTAS Author:Brooker, C (Ms Clare Brooker)
UTAS Author:Vaillancourt, RE (Professor Rene Vaillancourt)
ID Code:66863
Year Published:2010
Web of Science® Times Cited:12
Deposited By:Plant Science
Deposited On:2011-02-16
Last Modified:2011-05-12

Repository Staff Only: item control page