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Cyano bonded silica monolith - development of an in situ modification method for analytical scale columns


Soliven, A and Dennis, GR and Guiochon, G and Hilder, EF and Haddad, PR and Shalliker, RA, Cyano bonded silica monolith - development of an in situ modification method for analytical scale columns, Journal of Chromatography A, 1217, (39) pp. 6085-6091. ISSN 0021-9673 (2010) [Refereed Article]

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DOI: doi:10.1016/j.chroma.2010.07.052


This study investigates the synthesis and chromatographic behaviour of an analytical size cyanopropyl "cyano" bonded silica monolith. Surface modification was undertaken by treating a neat silica monolith with chloro(3-cyanopropyl)dimethyl silane in dry heptane over a two day period. The resulting monolith showed stability over the duration of the testing program that involved flushing the column with more than 2000 column volumes of mobile phase. Efficiency measurements before and after sylation verified that the integrity of the silica monolith itself was not affected by the modification process, the highest number of theoretical plates (N/m) using anisole was 81,650. A brief selectivity test was then undertaken to assess methylene selectivity and phenyl selectivity. Elemental analysis was used to determine the homogeneity of the carbon load throughout the monolithic bed, and was compared to two commercial C18 and one ‘self’ modified C18 silica monoliths. The development of the in situ modification is also discussed.

Item Details

Item Type:Refereed Article
Keywords:Silica monolithic columns; Cyano reversed phase; Carbon load axial homogeneity
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Hilder, EF (Professor Emily Hilder)
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:65184
Year Published:2010
Funding Support:Australian Research Council (DP0987318)
Web of Science® Times Cited:17
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2010-10-11
Last Modified:2011-05-12

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