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Identification of deletion mutants with improved performance under water-limited environments in rice (Oryza sativa L.)


Cairns, JE and Botwright Acuna, TL and Simborio, FA and Dimayuga, G and Lakshmi Praba, M and Leung, H and Torres, R and Lafitte, HR, Identification of deletion mutants with improved performance under water-limited environments in rice (Oryza sativa L.), Field Crops Research, 114, (1) pp. 159-168. ISSN 0378-4290 (2009) [Refereed Article]

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DOI: doi:10.1016/j.fcr.2009.07.019


Rice (Oryza sativa L.) is a semi-aquatic member of the grass family that is poorly adapted to dry environments and has greater sensitivity to water-deficits than other important cereals in this family. To increase productivity in aerobic or water-limited environments rice must overcome its adaptations to flooded environments. Deletion mutants offer an alternative genetic resource for improving drought tolerance. Almost 3500 IR64 deletion mutants were screened under vegetative and reproductive stage drought stress in the field and evaluated for leaf drying and/or grain yield. Seven novel conditional mutants of rice which showed gain of function through continued growth as drought stress developed compared to the wild type were identified. Mutant recovery rate was 0.1%. Further evaluation of putative drought mutants revealed that their average shoot biomass at maturity and grain yield per plant under stress exceeded those of the wild type by two-fold. Studies under controlled conditions confirmed mutants to have continued growth of both roots and shoots as drought developed compared to the wild type, and a tendency for greater water extraction. We propose that deletions in these mutants have affected a regulator of the highly conservative growth response common to irrigated lowland rice cultivars. Our results suggest that screening deletion mutants for performance under managed drought stress in the field could be a highly effective way to identify valuable genetic resources for improved drought response and aerobic adaptation in rice.

Item Details

Item Type:Refereed Article
Keywords:Rice; Oryza sativa L.; Drought stress; Deletion mutants; Aerobic adaptation
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Crop and pasture production
Research Field:Agronomy
Objective Division:Plant Production and Plant Primary Products
Objective Group:Grains and seeds
Objective Field:Rice
UTAS Author:Botwright Acuna, TL (Associate Professor Tina Acuna)
ID Code:64987
Year Published:2009
Web of Science® Times Cited:6
Deposited By:Agricultural Science
Deposited On:2010-09-21
Last Modified:2012-01-20
Downloads:1 View Download Statistics

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