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Electrokinetic chromatography and mass spectrometric detection using latex nanoparticles as a pseudostationary phase


Palmer, CP and Hilder, EF and Quirino, JP and Haddad, PR, Electrokinetic chromatography and mass spectrometric detection using latex nanoparticles as a pseudostationary phase, Analytical Chemistry, 82, (10) pp. 4046-4054. ISSN 0003-2700 (2010) [Refereed Article]

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Copyright Statement

Copyright 2010 American Chemical Society

DOI: doi:10.1021/ac902922u


The utility of novel latex nanoparticles as pseudostationary phases for electrokinetic chromatography with UV and mass spectrometric detection is demonstrated. The nanoparticles are synthesized using ab initio RAFT (reversible addition−fragmentation chain transfer) in emulsion polymerization, which yields small (63 nm) particles with a narrow size distribution, a hydrophobic core, and an ionic shell. The nanoparticles are shown to provide efficient and selective separations, with retention and separation selectivity dominated by hydrophobic interactions. The nanoparticles are highly retentive, such that they are effective at relatively low concentrations. Addition of the nanoparticles to the background electrolyte at these concentrations has a minor effect on the noise with UV detection, no measurable effect on the separation current, and minor effects on analyte ionization efficiency during electrospray ionization. The nanoparticles do not cause fouling or degradation of the electrospray−mass spectrometer interface even after several weeks of use. The combination of online sample preconcentration via sweeping and selective mass spectrometric detection yields low detection limits (10−16 ppb), particularly for more hydrophobic compounds.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Palmer, CP (Dr Christopher Palmer)
UTAS Author:Hilder, EF (Professor Emily Hilder)
UTAS Author:Quirino, JP (Associate Professor Lito Quirino)
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:63614
Year Published:2010
Funding Support:Australian Research Council (DP0666121)
Web of Science® Times Cited:33
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2010-05-17
Last Modified:2014-10-17

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