File(s) not publicly available
Processing of complex antigens and simple hapten-like molecules by epidermal Langerhans cells
journal contribution
posted on 2023-05-16, 09:59 authored by Gregory WoodsGregory Woods, Henderson, KG, Qu, MM, Muller, HKLangerhans cells (LCs) are antigen-presenting cells of the skin that trap small contact-sensitizing molecules and induce cutaneous hypersensitivity. LCs can present larger molecules but the mechanisms of processing have required investigation. A system combining in vitro culture of antigen with epidermal cells in the presence of inhibitors, followed by fixation and transfer of these antigen/drug treated epidermal cells to naive mice, was developed to investigate the steps of antigen processing. Langerhans cells undertake similar, but not identical, pathways for the processing of simple and complex molecules. Complex molecules such as trinitrophenyl conjugated to ovalbumin (TNP-OVA) were internalized Mice and modification required a chloroquine-sensitive proteolysis step and a cycloheximide-sensitive protein synthesis step. This modified product was actively recycled to the cell membrane as presentation was inhibited by blocking receptor translocation with either monensin or cytochalasin B. Small contact sensitizers such as trinitrophenyl did not undergo modification but required internalization and presentation was also inhibited by blocking receptor translocation.
History
Publication title
Leukocyte BiologyVolume
57Issue
6Pagination
891-896ISSN
0741-5400Department/School
Tasmanian School of MedicinePublisher
Federation Amer Soc Exp BiolPlace of publication
USARepository Status
- Restricted