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Sulfatide-tenascin interaction mediates binding to the extracellular matrix and endocytic uptake of liposomes in glioma cells
journal contribution
posted on 2023-05-17, 01:43 authored by Shao, K, Hou, Q, Gob, ML, Duan, W, Cheung, NS, Feng, SS, Wong, KP, Yoram, A, Zhang, W, Huang, Z, Li, QTTenascin-C is an extracellular matrix glycoprotein, whose expression is highly restricted in normal adult tissues, but markedly up-regulated in a range of tumors, and therefore serves as a potential receptor for targeted anticancer drug or gene delivery. We describe here a liposomal carrier system in which the targeting ligand is sulfatide. Experiments with tenascin-C-expressing glioma cells demonstrated that binding of liposomes to the extracellular matrix relied essentially on the sulfatide-tenascin-C interaction. Following binding to the extracellular matrix, the sulfatide-containing liposomes were internalized via both caveolae/lipid raft- and clathrin-dependent pathways, which would ensure direct cytoplasmic release of the cargoes carried in the liposomes. Such natural lipid-guided intracellular delivery targeting at the extracellular matrix glycoproteins of tumor cells thus opens a new direction for development of more effective anticancer chemotherapeutics in future.
History
Publication title
Cellular and Molecular Life SciencesVolume
64Issue
4Pagination
506-515ISSN
1420-682XDepartment/School
Menzies Institute for Medical ResearchPublisher
Birkhauser Verlag AgPlace of publication
Viadukstrasse 40-44, Po Box 133, Basel, Switzerland, Ch-4010Repository Status
- Restricted