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Identification of a new noradrenaline induced gene in the rat heart by differential messenger-RNA display
journal contribution
posted on 2023-05-16, 09:58 authored by Venkataraman, R, Presser, JL, Rene VaillancourtRene Vaillancourt, Adrian WestAdrian WestObjective: Noradrenaline treatment of animals results in postnatal hypertrophy of the heart. This process requires many qualitative and quantitative changes in gene expression; however, the identities of the key regulatory genes which modulate the process are not known. The aim of this study was to investigate whether a recently developed technique, differential display, could provide a new route to the identification and characterisation of these critical genes. Methods: The technique of differential display was modified for use on cardiac RNA samples and the expression of clones identified by this approach was characterised by northern analysis. Results: Differential display was successfully adapted to the study of noradrenaline induced cardiac gene expression. A previously unsuspected gene was identified, the expression of which appears to be strongly modified during the onset of this process. Conclusions: Differential display offers the potential to identify and clone many of the genes critically important in regulation of growth of the mammalian heart.
History
Publication title
Cardiovascular ResearchVolume
29Issue
4Pagination
490-494ISSN
0008-6363Department/School
Tasmanian School of MedicinePublisher
Elsevier Science BvPlace of publication
Po Box 211, Amsterdam, Netherlands, 1000 AeRepository Status
- Restricted