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Controlling influence of phosphocitrate in vitro and in vivo on calcium oxalate crystal formation and growth


Sallis, JD and Parry, NFG and Meehan, JD and Kamperman, H and Anderson, M, Controlling influence of phosphocitrate in vitro and in vivo on calcium oxalate crystal formation and growth, Scanning Microscopy, 9, (1) pp. 127-136. ISSN 0891-7035 (1995) [Refereed Article]


Calcium oxalate (CaOx) crystallization in the presence of phosphocitrate (PC) was studied by both in vitro and in vivo techniques. Crystals of the monohydrate (COM) and the dihydrate (COD) forms were generated under controlled conditions in a silica gel matrix. Our data indicated only COD crystals formed when PC was present, inferring that the COD to COM transformation was being impeded. COD crystals were smaller in size than controls and there was evidence of interpenetral twinning. An in vivo study using a rat bladder implant model noted similar findings. Scanning electron microscopy (SEM) revealed that implants recovered from PC treated rats had primarily COD crystals deposited, whereas both the surface and inner layers of encrusted implants from normal rats contained predominantly COM crystals. Infrared (IR) analysis confirmed the visual findings indicating quantitatively that there was a higher proportion of COD present on the implants recovered from the treated rats than in the controls. It is concluded that although total CaOx crystallization cannot be eliminated by PC, its action could assist in reducing the harmful nature of such crystallites in the urine.

Item Details

Item Type:Refereed Article
Research Division:Health Sciences
Research Group:Health services and systems
Research Field:Health services and systems not elsewhere classified
Objective Division:Health
Objective Group:Other health
Objective Field:Other health not elsewhere classified
UTAS Author:Sallis, JD (Dr John Sallis)
UTAS Author:Parry, NFG (Mr Nathan Frank Glynn Parry)
UTAS Author:Meehan, JD (Mr John David Meehan)
UTAS Author:Kamperman, H (Mr Harold Kamperman)
ID Code:5867
Year Published:1995
Web of Science® Times Cited:14
Deposited By:Biochemistry
Deposited On:1995-08-01
Last Modified:2011-11-28

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