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Identification of gibberellin A4 in Pisum sativum L. and the effects of applied gibberellins A9, A4, A5 and A3 on the le mutant

journal contribution
posted on 2023-05-16, 09:54 authored by Poole, AT, John RossJohn Ross, Lawrence, NL, James ReidJames Reid
Gibberellin A4 (GA4) was identified for the first time in the garden pea (Pisum sativum) L.), by gas chromatography-mass spectrometry. However, in wild-type shoots the level of GA4 was only about 6% of the level of GA1, and it is therefore unlikely that GA4 plays a major role per se in the control of pea stem elongation. In shoots of the le mutant, GA4 was not detected, while the level of GA9 was approximately twice that found in the wild-type. The le mutation also markedly reduced the elongation response to applied GA9. It appears, therefore, that in Pisum the le mutation blocks the 3β-hydroxylation of GA9 to GA4, in addition to the 3β-hydroxylation of GA20 to GA1. In contrast, the le mutation did not reduce the response to applied GA5, suggesting the step GA5 to GA3 is not catalysed by the enzyme controlled by the Le gene. The step GA5 to GA3 was confirmed in peas by metabolite analysis after treatment with deuterated GA5. © 1995 Kluwer Academic Publishers.

History

Publication title

Plant Growth Regulation

Volume

16

Pagination

257-262

ISSN

0167-6903

Department/School

School of Natural Sciences

Publisher

Kluwer Academic Publ

Place of publication

Van Godewijckstraat 30, Dordrecht, Netherlands, 3311 Gz

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the environmental sciences

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