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Identification of gibberellin A4 in Pisum sativum L. and the effects of applied gibberellins A9, A4, A5 and A3 on the le mutant
journal contribution
posted on 2023-05-16, 09:54 authored by Poole, AT, John RossJohn Ross, Lawrence, NL, James ReidJames ReidGibberellin A4 (GA4) was identified for the first time in the garden pea (Pisum sativum) L.), by gas chromatography-mass spectrometry. However, in wild-type shoots the level of GA4 was only about 6% of the level of GA1, and it is therefore unlikely that GA4 plays a major role per se in the control of pea stem elongation. In shoots of the le mutant, GA4 was not detected, while the level of GA9 was approximately twice that found in the wild-type. The le mutation also markedly reduced the elongation response to applied GA9. It appears, therefore, that in Pisum the le mutation blocks the 3β-hydroxylation of GA9 to GA4, in addition to the 3β-hydroxylation of GA20 to GA1. In contrast, the le mutation did not reduce the response to applied GA5, suggesting the step GA5 to GA3 is not catalysed by the enzyme controlled by the Le gene. The step GA5 to GA3 was confirmed in peas by metabolite analysis after treatment with deuterated GA5. © 1995 Kluwer Academic Publishers.
History
Publication title
Plant Growth RegulationVolume
16Pagination
257-262ISSN
0167-6903Department/School
School of Natural SciencesPublisher
Kluwer Academic PublPlace of publication
Van Godewijckstraat 30, Dordrecht, Netherlands, 3311 GzRepository Status
- Restricted