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Column selection for comprehensive multidimensional ion chromatography


Shellie, RA and Tyrrell, EC and Pohl, CA and Haddad, PR, Column selection for comprehensive multidimensional ion chromatography, Journal of Separation Science, 31, (19) pp. 3287-3296. ISSN 1615-9306 (2008) [Refereed Article]

DOI: doi:10.1002/jssc.200800286


This paper discusses the selection of ion chromatography (IC) columns for use in comprehensive multidimensional ion chromatography (IC×IC). First, a single number was determined for a wide range of anions (one number for each anion) using the linear solvent strength model. These numbers were then used to compare the column selectivity characteristics for five different columns. Principal component analysis was used to illustrate selectivity differences between columns. Dionex AS16 and AS20 columns were selected for use in the development of an IC×IC method for the separation of ten anions. To achieve the required speed of analysis in both the first and second separation dimensions, custom column lengths were packed in-house. The use of an eluent suppressor between the first and second columns permits a relatively low flow ratio regime of only <1:20 in the first and second dimensions, respectively, which reduces dilution effects common in comprehensive multidimensional LC. Selection of the second dimension eluent conditions was aided by the development of a spreadsheet based on the linear solvent strength model. © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Shellie, RA (Associate Professor Robert Shellie)
UTAS Author:Tyrrell, EC (Dr Eadaoin Tyrrell)
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:55255
Year Published:2008
Web of Science® Times Cited:17
Deposited By:Chemistry
Deposited On:2009-03-06
Last Modified:2014-11-21

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