File(s) under permanent embargo
Toward sol-gel electrochromatographic separations on a chip
journal contribution
posted on 2023-05-16, 22:55 authored by Morishima, K, Bennett, BD, Dulay, MT, Joselito Quirino, Zare, RNA porous photopolymerized sol-gel (PSG) monolith was synthesized in the separation channel of a borosilicate glass chip via UV irradiation (5 min) of a mixture of 3-methacryloxypropyltrimethoxysilane, an acid catalyst, a porogen, and a photoinitiator. The PSG monolith adhered strongly to the chemically untreated channel walls. The chip was fabricated using standard lithography procedures to give channels that are 35-m deep and 90-m wide. Masking the other channels defined the 4.7-cm PSG section in the separation channel. Two dyes Coumarin 314 and 510 were successfully separated within baseline resolution in 225 s when fluorescent detection occurred immediately after the PSG section. The separation time was reduced to 80 s with little loss in resolution by detecting the dyes 1.2 cm from the front of the PSG monolith.
History
Publication title
Journal of Separation ScienceVolume
25Issue
15-17Pagination
1226-1230ISSN
1615-9314Department/School
School of Natural SciencesPublisher
WileyPlace of publication
USARights statement
The definitive published version is available online at: http://interscience.wiley.comRepository Status
- Restricted