2008_Redd,_Jarman,_Frusher_&_Johnson_Bull_Ent_Res.pdf (93.31 kB)
A molecular approach to identify prey of the southern rock lobster
journal contribution
posted on 2023-05-16, 21:48 authored by Redd, KS, Jarman, SN, Stewart FrusherStewart Frusher, Craig JohnsonCraig JohnsonWe demonstrate the use of molecular techniques to detect specific prey consumed by the southern rock lobster (Jasus edwardsii). A quick and non-lethal method was used to collect rock lobster faecal material and a molecular protocol was employed to isolate prey DNA from faecal samples. The isolated DNA was amplified using the polymerase chain reaction (PCR) with PCR primers designed to target specific prey items. Feeding experiments determined that DNA from black-lipped abalone (Haliotis rubra) and sea urchins (Centrostephanus rodgersii and Heliocidaris erythrogramma) can be detected in rock lobster faecal samples within seven hours and remains present for up to 60 h after ingestion. Copyright © 2008 Cambridge University Press.
History
Publication title
Bulletin of Entomological ResearchVolume
98Pagination
233-238ISSN
0007-4853Department/School
School of Natural SciencesPublisher
Cambridge University PressPlace of publication
United KingdomRepository Status
- Restricted