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Identification and expression of natriuretic peptide receptor type-A and -B mRNA in freshwater and seawater rainbow trout

journal contribution
posted on 2023-05-16, 21:02 authored by Nankervis, S, Powell, MD, McLeod, J, Toop, T
Natriuretic peptide receptors mediate the physiological response of natriuretic peptide hormones. One of the natriuretic peptide receptor types is the particulate guanylyl cyclase receptors, of which there are two identified: NPR-A and NPR-B. In fishes, these have been sequenced and characterized in eels, medaka, and dogfish shark (NPR-B only). The euryhaline rainbow trout provides an opportunity to further pursue examination of the system in teleosts. In this study, partial rainbow trout NPR-A-like and NPR-B-like mRNA sequences were identified via PCR and cloning. The sequence information was used in real-time PCR to examine mRNA expression in a variety of tissues of freshwater rainbow trout and rainbow trout acclimated to 35 parts per thousand seawater for a period of 10 days. In the excretory kidney and posterior intestine, real-time PCR analysis showed greater expression of NPR-B in freshwater fish than in those adapted to seawater; otherwise, there was no difference in the expression of the individual receptors in fresh water or seawater. In general, the expression of the NPR-A and NPR-B type receptors was quite low. These findings indicate that NPR-A and NPR-B mRNA expression is minimally altered under the experimental regime used in this study. © 2006 Springer-Verlag.

History

Publication title

Journal of Comparative Physiology B: biochemical, systemic, and environmental physiology

Volume

177

Pagination

259-267

ISSN

0174-1578

Department/School

Institute for Marine and Antarctic Studies

Publisher

Springer Netherlands

Place of publication

United States

Repository Status

  • Restricted

Socio-economic Objectives

Fisheries - aquaculture not elsewhere classified

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