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Enhanced resistance to common scab of potato through somatic cell selection in cv. Iwa with the phytotoxin thaxtomin A


Wilson, CR and Luckman, G and Tegg, RS and Yuan, ZQ and Wilson, AJ and Eyles, A and Conner, AJ, Enhanced resistance to common scab of potato through somatic cell selection in cv. Iwa with the phytotoxin thaxtomin A, Plant Pathology, 58, (1) pp. 137-144. ISSN 0032-0862 (2009) [Refereed Article]

DOI: doi:10.1111/j.1365-3059.2008.01903.x


A somatic cell selection approach, developed using thaxtomin A as a positive selection agent, recovered 113 potato cell colonies of cv. Iwa from which 39 regenerated plants within a single subculture period. In glasshouse pathogenicity trials, 13 of these regenerated variants exhibited significantly greater resistance to common scab disease than the unselected parent cultivar, both in mean tuber surface coverage with lesions and the proportion of disease free tubers. The best variant had on average an 85-86% lower disease score (or 91-92% less estimated tuber surface cover) than the unselected parent cultivar. The disease-resistant variants varied in their response to thaxtomin A in detached leaflet and tuber slice bioassays. This suggested that the cell selection procedure and thaxtomin A screening had limited success in selecting for toxin tolerance, and that thaxtomin A tolerance was not necessarily the driving factor for induction of disease resistance observed. Variation in relative necrosis scores between leaflet and tuber assays for individual variants possibly suggests differential expression of thaxtomin A tolerance in the different tissues. Yield estimates from the glasshouse grown potatoes showed the majority of disease-resistant variants had tuber yields equivalent to the unselected parent cultivar. This suggests that selection for disease resistance was not associated with negative tuber yield attributes and that these variants may have commercial merit and are worthy of further agronomic testing. © 2008 The Authors.

Item Details

Item Type:Refereed Article
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Horticultural production
Research Field:Horticultural crop protection (incl. pests, diseases and weeds)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural crops
Objective Field:Field grown vegetable crops
UTAS Author:Wilson, CR (Professor Calum Wilson)
UTAS Author:Luckman, G (Mr Gregory Luckman)
UTAS Author:Tegg, RS (Dr Robert Tegg)
UTAS Author:Yuan, ZQ (Dr Zi Yuan)
UTAS Author:Wilson, AJ (Mrs Amy Wilson)
UTAS Author:Eyles, A (Dr Alieta Eyles)
ID Code:49604
Year Published:2009
Web of Science® Times Cited:27
Deposited By:Agricultural Science
Deposited On:2008-08-01
Last Modified:2012-03-05

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