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Latex-functionalized monolithic columns for the separation of carbohydrates by micro anion-exchange chromatography

journal contribution
posted on 2023-05-16, 20:04 authored by Emily HilderEmily Hilder, Svec, F, Frechet, JMJ
A novel stationary phase for micro ion chromatography has been prepared by coating a porous poly(butyl methacrylate-co-ethylene dimethacrylate-co-2- acrylamido-2-methyl-1-propanesulfonic acid) monolith with quaternary amine-functionalized latex particles via simple electrostatic binding. This stationary phase enabled the separation of saccharides in the mobile phase with a high-pH value consisting of aqueous ammonia solution in anion-exchange mode using evaporative light scattering for detection. Effects of both porous properties of the monolithic stationary phase and chromatographic conditions on the separation ability were studied. Under optimized conditions, an efficient separation of seven saccharides was achieved in less than 10 min. The stationary phase also enables the separation of saccharides obtained by the enzymatic hydrolysis of corn starch. © 2004 Elsevier B.V. All rights reserved.

History

Publication title

Journal of Chromatography A

Volume

1053

Issue

1-2

Pagination

101-106

ISSN

0021-9673

Department/School

School of Natural Sciences

Publisher

Elsevier

Place of publication

Amsterdam

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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