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Genetic Screening for Prey in the Gut Contents from a Giant Squid (Architeuthis sp.)
journal contribution
posted on 2023-05-16, 17:38 authored by Bruce Deagle, Jarman, SN, Pemberton, D, Gales, NJGiant squids (Architeuthis sp.) remain mysterious; they have evaded observation and are rarely taken from their deep sea habitat. Information on the diet of Architeuthis is scarce due to the limited number of specimens with morphologically recognizable remains in their digestive tracts. We explored the use of polymerase chain reaction (PCR)-based methods for detection of DNA in the prey remains and amorphous slurry from an Architeuthis gut sample. The DNA region amplified varied in size, allowing separation of fish and squid components. Sequence comparisons identified fish prey as Macruronus novaezelandiae. Isolation of Architeuthis DNA from an ingested tentacle and the presence of chitin fragments indicate cannibalism occurs in giant squid. Denaturing gradient gel electrophoresis was used to screen for less common DNA types, revealing a high frequency of PCR-generated false alleles, but no additional prey species. © The American Genetic Association. 2005. All rights reserved.
History
Publication title
Journal of HeredityVolume
96Issue
4Pagination
417-423ISSN
0022-1503Department/School
School of Natural SciencesPublisher
Oxford Univ PressPlace of publication
USARepository Status
- Restricted