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Functional and metabolic evidence for two different vanilloid (VN1 and VN2) receptors in perfused rat hindlimb

journal contribution
posted on 2023-05-16, 09:40 authored by Colquhoun, EQ, Eldershaw, TPD, Bennett, KL, Hall, JL, Dora, KA, Michael ClarkMichael Clark
Vanilloid spice principles, including capsaicin, stimulate vasoconstriction in the rat hindlimb perfused at constant flow and, depending on dose, either stimulate or inhibit oxygen consumption by this vascular bed. We now present metabolic and functional evidence for two different vanilloid (VN 1 and VN 2) receptor types. These receptors can be distinguished on the basis of their differing agonist affinity for capsacin, their different calcium and oxygen dependencies for inducing vasoconstriction, and whether they stimulate, or inhibit, oxygen consumption. The higher affinity vanilloid receptor, VN 1 can be distinguished on the basis of initiating vasoconstriction at low doses of capsaicin and stimultaneously stimulating oxygen consumption. Its apparent biological function is dependent on the presence of oxygen and external calcium. In contrast, the lower affinity receptor, VN 2 induces vasoconstriction associated with inhibition of oxygen consumption. Its vasoconstriction action can occur independently of either external calcium ions, or the presence of oxygen in the perfusate.

History

Publication title

Life Sciences

Volume

57

Pagination

91-102

ISSN

0024-3205

Department/School

Tasmanian School of Medicine

Publisher

Pergamon-Elsevier Science Ltd

Place of publication

The Boulevard, Langford Lane, Kidlington, Oxford, England, Ox5 1Gb

Repository Status

  • Restricted

Socio-economic Objectives

Other health not elsewhere classified

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