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Analysis of microbial hydrocarbon degradation using TLC-FID


Cavanagh, JE and Juhasz, AL and Nichols, PD and Franzmann, PD and McMeekin, TA, Analysis of microbial hydrocarbon degradation using TLC-FID, Journal of Microbiological Methods, 22, (2) pp. 119-130. ISSN 0167-7012 (1995) [Refereed Article]

DOI: doi:10.1016/0167-7012(94)00065-F


Biodegradation of the hydrocarbons, tetradecane and phenanthrene, by mixed and pure cultures of Antarctic bacteria was measured using thin layer chromatography-flame ionisation detection (TLC-FID). Losses of up to 65% of tetradecane and complete degradation of phenanthrene with concurrent increases in bacterial biomass, measured by polar lipid concentration, were observed. TLC-FID has advantages over other analytical techniques used to monitor hydrocarbon biodegradation by enabling a number of samples to be replicated and run simultaneously in a short period of time. TLC-FID analysis enables three samples to be run in triplicate and analysed in 45 min. Analysis time may be further reduced if only one compound class is present in the samples. The TLC-FID system can detect concentrations as low as 0.1 μg/μl, as applied on the chromarods, with a reproducibility of ± 10%. Biodegradation of low molecular weight hydrocarbons, while not accurately quantitated using TLC-FID, could be qualitatively assessed using this system.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and cell biology
Research Field:Analytical biochemistry
Objective Division:Environmental Management
Objective Group:Other environmental management
Objective Field:Other environmental management not elsewhere classified
UTAS Author:Cavanagh, JE (Ms Joanne Elizabeth Cavanagh)
UTAS Author:Juhasz, AL (Mr Albert Laszlo Juhasz)
UTAS Author:Nichols, PD (Dr Peter Nichols)
UTAS Author:Franzmann, PD (Dr Franzmann)
UTAS Author:McMeekin, TA (Professor Thomas McMeekin)
ID Code:3779
Year Published:1995
Web of Science® Times Cited:8
Deposited By:Agricultural Science
Deposited On:1995-08-01
Last Modified:2011-08-22

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