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Measurement of Lung Diffusing Capacity and Functional Residual Capacity in Lambs During the First Postnatal Month


Johns, DP and Jakubowska, AE and McCrabb, GJ and Harding, R, Measurement of Lung Diffusing Capacity and Functional Residual Capacity in Lambs During the First Postnatal Month, Pediatric Pulmonology, 17 pp. 347-353. ISSN 8755-6863 (1994) [Refereed Article]

DOI: doi:10.1002/ppul.1950170602


The diffusing capacity of the lung for carbon monoxide (DLCO) is an important index of lung function but is not easily measured in spontaneously breathing animals with small lung volumes. Our aim was to devise a simple rebreathing method that would allow us to make serial measurements of DLCO in spontaneously breathing lambs during their first month after birth. By adding He to the rebreathing gas mixture, we were also able to measure functional residual capacity (FRC), enabling us to normalize DLCO with respect to FRC. We have compared FRC measured by the rebreathing technique with that measured by a closed‐circuit helium dilution method (FRCcc). Using the rebreathing method we found highly significant positive correlations between DLCO and body weight (r = 0.70, P < 0.001) and between FRC and body weight (r = 0.79, P < 0.001). There was no significant change in DLCO/FRC over the first postnatal month; the mean value was 8.1± 0.6 ML/min/mmHg/mL. Rebreathing FRC was highly correlated with FRC, (r = 0.88, P < 0.001), but was lower than FRCcc by about 18%. In normal lambs DLCO and FRC, but not DLCO/FRC, increased during the first month after birth, suggesting that the increase in DLCO parallels lung growth. We conclude that the modified rebreathing method is suitable for measuring DLCO in small uncooperative spontaneously breathing animals. Pediatr Pulmonol. 1994; 17:347"353. © 1994 Wiley‐Liss, Inc. Copyright © 1994 Wiley‐Liss, Inc., A Wiley Company

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Cardiovascular medicine and haematology
Research Field:Respiratory diseases
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Johns, DP (Associate Professor David Johns)
ID Code:35869
Year Published:1994
Web of Science® Times Cited:9
Deposited By:Medicine
Deposited On:2005-09-12
Last Modified:2005-09-12

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