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Differential effects of temperature and maturity stage on hepatic estrogen receptor characteristics of Atlantic salmon

journal contribution
posted on 2023-05-16, 16:12 authored by Watts, M, Pankhurst, NW, King, H, Dominic GeraghtyDominic Geraghty
In order to determine if elevated temperature during vitellogenesis had a detrimental effect on hepatic estrogen receptors of Atlantic salmon (Salmo salar), 3H-estradiol saturation binding analysis, using one- and two-site binding models, was carried out on extracts of hepatic cytosols from fish held at 14, 18 or 22°C over the austral period of peak vitellogenesis (February to April). With one-site binding analysis, no temperature related difference in either receptor affinity (Kd) or number (B max) was found at each sampling point, but there was an apparent decrease in both affinity and number at each temperature over the period of the study. However, some analyses, notably at 22°C during February, were best described using a two-site binding model. At this temperature and time, there was a clear separation of binding affinity into high and low components (K d=0.67±S.E. 0.05 and 20±S.E. 5.6 nM, respectively) (n=4), which suggests that February was a critical time of temperature related hepatic sensitivity to estrogen. These results support those of other studies where we found that February was also a sensitive time with respect to temperature impairment of in vitro follicular estrogen synthesis, and the greatest period of in vivo temperature sensitivity. © 2005 Elsevier Inc. All rights reserved.

History

Publication title

Comparative Biochemistry and Physiology. Part A: Molecular & Integrated Physiology

Volume

140

Pagination

377-383

ISSN

1095-6433

Department/School

Institute for Marine and Antarctic Studies

Publisher

Elselvier Inc.

Place of publication

United States

Repository Status

  • Restricted

Socio-economic Objectives

Fisheries - aquaculture not elsewhere classified

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