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The opsonising activity of a pentraxin-like protein isolated from snapper (Pagrus auratus, Sparidae) serum


Cook, MT and Hayball, PJ and Nowak, BF and Hayball, JD, The opsonising activity of a pentraxin-like protein isolated from snapper (Pagrus auratus, Sparidae) serum, Developmental and Comparative Immunology, 29, (8) pp. 703-712. ISSN 0145-305X (2005) [Refereed Article]

DOI: doi:10.1016/j.dci.2004.05.009


In mammals the pentraxins, C-reactive protein (CRP) and serum amyloid P component (SAP) are important components of the immune response. Although pentraxins have been isolated from a number of fish species few studies detail their functional immunological role. In this paper we report the establishment of a flow cytometry based assay to measure the phagocytic activity of isolated snapper head kidney leukocytes (HKLs). This assay was then used to examine the ability of a pentraxin-like protein isolated from the serum of snapper (P. auratus) (Sn-PLP) to act as an opsonin. Incubation of snapper head kidney leukocytes (HKL) with FITC-labelled beads resulted in uptake of these particles by approximately 35% of HKLs. Incubation of beads with Sn-PLP enhanced phagocytosis by snapper HKLs in a dose-dependant manner. Enhanced phagocytosis could be inhibited by addition of a rabbit anti-Sn-PLP antibody suggesting that Sn-PLP may act as a ligand for a HKL cell surface receptor. This study provides further evidence toward a functional role for pentraxins in the host defence repertoire of fish. © 2005 Elsevier Ltd. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Fisheries sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Fisheries - aquaculture not elsewhere classified
UTAS Author:Nowak, BF (Professor Barbara Nowak)
ID Code:32834
Year Published:2005
Web of Science® Times Cited:16
Deposited By:TAFI - Aquaculture
Deposited On:2005-08-01
Last Modified:2006-04-01

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