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Rapid, high-throughput extraction of bacterial genomic DNA from selective-enrichment culture media
journal contribution
posted on 2023-05-16, 13:15 authored by Wilson, TK, Carson, JAims: To create a fast, sensitive and inexpensive high-throughput method for the extraction of bacterial genomic DNA from selective-enrichment culture media. Methods and Results: Lysis of bacteria was achieved using guanidinium isothiocyanate, and DNA was extracted using 96-well glass microfibre filtration plates. Extraction-PCR detected the presence of 1 cfu Yersinia ruckeri and 16 cfu Lactococcus garvieae 200 μl-1 sample of selective-enrichment medium. Conclusions: An efficient method for high-throughput extraction of bacterial genomic DNA from selective-enrichment culture media was achieved. Significance and Impact of the Study: This method enables detection of covert bacterial infections in fish. The simultaneous extraction of large numbers of samples allows for its use in bacterial monitoring programmes and quarantine.
History
Publication title
Letters in Applied MicrobiologyVolume
32Issue
5Pagination
326-330ISSN
0266-8254Department/School
Institute for Marine and Antarctic StudiesPublisher
Blackwell Science LtdPlace of publication
Oxford, UKRepository Status
- Restricted