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Identification of two down-regulated genes in rat liver allografts by mRNA differential display


Lin, YC and Goto, S and Pan, TL and Hong, YR and Lin, CL and Lord, RJ and Chiang, KC and Lai, CY and Tseng, HP and Hsu, LW and Iwashita, S and Kitano, S and Chen, CL, Identification of two down-regulated genes in rat liver allografts by mRNA differential display, Transplant International, 14, (3) pp. 153-158. ISSN 0934-0874 (2001) [Refereed Article]

DOI: doi:10.1111/j.1432-2277.2001.tb00034.x


Total RNA differential display (DD) using random primers was performed for rat orthotopic liver transplantation (OLT) models. DA (RT1a) donor livers were transplanted into DA, PVG (RT1c), and LEW (RT1l) recipients: (1) syngeneic OLT (DA-DA): no rejection occurs; (2) allogeneic OLT (DA-PVG): rejection occurs, but is naturally overcome without immuno-suppression; (3) allogeneic OLT (DA-LEW): animals die of acute rejection within 14 days. cDNA was isolated from selected bands, re-amplified for sequencing, and confirmed by Northern blots. Two down-regulated genes were observed in day-7 allogeneic OLT livers (DA-PVG, DA-LEW), while they were consistently expressed in day-7 syngeneic OLT (DA-DA) livers. These two genes were identified as α-glutathione sulfotransferase (α-GST) Ya gene and estrogen sulfotransferase (EST), respectively. Northern blots confirmed that their expression was down-regulated in OLT (DA-PVG) livers on days 7-26 and gradually restored. The mRNA expression of GST and EST may be good markers to predict rejection or induction of tolerance.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Medical biochemistry and metabolomics
Research Field:Medical biochemistry - nucleic acids
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Lord, RJ (Dr Roger Lord)
ID Code:22930
Year Published:2001
Web of Science® Times Cited:3
Deposited By:Surgery
Deposited On:2001-08-01
Last Modified:2011-08-03

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