University of Tasmania
Browse

File(s) not publicly available

Isolation and partial characterisation of immunoglobulin from southern bluefin tuna Thunnus maccoyii Castelnau

journal contribution
posted on 2023-05-16, 13:07 authored by Watts, M, Munday, BL, Burke, CM
Specific and total serum immunoglobulins were extracted by immunoaffinity, mannan-binding protein and Protein A affinity chromatography from southern bluefin tuna (Thunnus maccoyii Castelnau) immunised with rabbit IgG, and from non-immunised southern bluefin tuna. SDS-PAGE in 10% reducing gels revealed two heavy chains with molecular weights of approximately 74·6±1·3kDa and 71·2±0·9kDa, and two light chains with molecular weights of approximately 29±1·2kDa and 28±1·0kDa. Under non-reducing, but denaturing, conditions in 4% and 5% SDS-PAGE gels, a high molecular weight and a low molecular weight fraction were demonstrated. By gel filtration using Sephacryl HR300 a molecular weight of 845kDa, consistent with a tetramer, was obtained for the high molecular weight fraction, and a molecular weight of 168kDa, consistent with a monomer, was obtained for the low molecular weight fraction. The extinction coefficient at A280for the purified immunoglobulin (Ig) was determined to be 1·24. Tuna α-rabbit IgG Ig was reactive with all non-reduced mammalian IgG antigens tested, suggesting that common conformational antigenic determinants were recognised. © 2001 Academic Press.

History

Publication title

Fish & Shellfish Immunology

Volume

11

Issue

6

Pagination

491-503

ISSN

1050-4648

Department/School

Institute for Marine and Antarctic Studies

Publisher

Academic Press

Place of publication

United Kingdom

Repository Status

  • Restricted

Socio-economic Objectives

Fisheries - aquaculture not elsewhere classified

Usage metrics

    University Of Tasmania

    Categories

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC