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Modelling of migration behaviour of inorganic anions in ion-exchange capillary electrochromatography

journal contribution
posted on 2023-05-16, 12:58 authored by Michael BreadmoreMichael Breadmore, Emily HilderEmily Hilder, Miroslav MackaMiroslav Macka, Avdalovic, N, Paul HaddadPaul Haddad
A theoretical model to explain the observed mobility of inorganic anions in capillary electrochromatography (CEC) using ion-exchange (IE) stationary phases has been derived. The model divides contributions to the observed mobility of an analyte ion into capillary electrophoretic (CE) and IE components. The CE component includes the influence of varying the ionic strength of the background electrolyte on the electrophoretic mobility of the analyte, while the IE component accounts for the variation in retention of the analyte ion caused by changing the composition of the background electrolyte. The model was verified using a mixture of UV-absorbing inorganic ions in electrolytes of differing eluotropic strength in both packed and open-tubular CEC systems, with excellent agreement (r2>0.98) for both systems. Values of constants in the model equation determined by nonlinear regression were used to estimate the relative strengths of the interactions of different analytes with the stationary phase and these were found to agree well with elution orders observed in conventional IE chromatography.

History

Publication title

Electrophoresis

Volume

22

Pagination

503-510

ISSN

0173-0835

Department/School

School of Natural Sciences

Publisher

Wiley-VCH Verlag GmbH

Place of publication

Berlin, Germany

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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