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Development and validation of a new dot blot test for the detection of Paramoeba pemaquidensis (Page) in fish

journal contribution
posted on 2023-05-16, 12:44 authored by Douglas-Helders, GM, Carson, J, Howard, T, Barbara NowakBarbara Nowak
In this study, the development of a dot blot assay to assess amoebic gill disease (AGD) using non-lethal gill mucus samples is described and its performance validated by comparing the assay with the indirect fluorescent antibody test (IFAT), the 'gold standard' test. The agreement between the two tests was high, with a positive predictive value of 95% and negative predictive value of 93%, with a corrected κ value of 0.88. The sensitivity and specificity of the test were 97 and 91%, respectively. The dot blot is both sensitive and specific for Paramoeba pemaquidensis and is formatted so that large numbers of samples can be conveniently analysed.

History

Publication title

Journal of Fish Diseases

Volume

24

Issue

5

Pagination

273-280

ISSN

0140-7775

Department/School

Institute for Marine and Antarctic Studies

Publisher

Blackwell Science Ltd

Place of publication

Oxford, England

Repository Status

  • Restricted

Socio-economic Objectives

Fisheries - aquaculture not elsewhere classified

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