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Simple gill Smear Staining for Diagnosis of Amoebic Gill Disease

journal contribution
posted on 2023-05-16, 11:45 authored by Zilberg, D, Barbara NowakBarbara Nowak, Carson, J, Wagner, T
Amoebic gill disease (AGD) caused by a Paramoeba spp. is the major disease affecting cultured Atlantic salmon in Tasmania. Primary diagnosis of the disease on the farm relies on gross morphology, and it is then confirmed in the laboratory with an immunofluorescence antibody test (IFAT). In this study we evaluated the potential use of a rapid method for the detection of AGD using a Quick Dip* stain and compared it to the established IFAT. Quick Dip* staining revealed 96% agreement with IFAT in the determination whether a sample is positive or negative for AGD, with sensitivity and specificity values of 88.23% and 92.85%, respectively and a kappa value of 0.7628. Mean number of Paramoeba spp. per field of view positively correlated in the two methods (r=0.972), although it was significantly greater when IFAT was used. Quick Dip* stained Paramoeba spp. in a mucus smear is identified by its morphological features. It measures 15 to 20 μm, appearing dark blue with a darker blue and purple-stained internal organelles. The study suggests that Quick Dip* can be used as a fast method for the diagnosis of AGD.

History

Publication title

Bulletin of European Association of Fish Pathologists

Volume

19

Issue

5

Pagination

186-189

ISSN

0108-0288

Department/School

Institute for Marine and Antarctic Studies

Publisher

European Association of Fish Pathologists

Place of publication

Aberdeen, Scotland

Repository Status

  • Restricted

Socio-economic Objectives

Fisheries - aquaculture not elsewhere classified

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