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Poster 107 - Modelling the effect of Amyloid Beta in oligodendrocyte differentiation and maturation

Citation

Dwyer, ST and Leung, JYK and Kirkcaldie, M and Vickers, J and King, A, Poster 107 - Modelling the effect of Amyloid Beta in oligodendrocyte differentiation and maturation, Australasian Neuroscience Society Annual Scientific Meeting 2016, 4-7 December, Hobart, Tasmania (2016) [Conference Extract]


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Abstract

Oligodendrocytes (OL) are a supporting cell in the brain, whose main function is to produce myelin. Myelin acts as insulation to the axons, allowing rapid transmission of action potential as well as maintaining their health and structure. The loss of myelin has been reported in Alzheimer’s disease (AD), one of the most commonly occurring forms of dementia. The reported myelin loss has often been considered as a secondary event, which occurs as a result of the loss of axons, however, recent studies have shown evidence that myelin loss could be an independent event in AD, resulting from the toxicity of beta-amyloid. To determine the effect of beta-amyloid on oligodendrocytes, we first established and characterized a primary culture model to grow oligodendrocytes to specific stages of differentiation (OPC, ImmatureOL, Mature-OL) using stage-specific media and following a recently published protocol. Immunocytochemical analysis was used to characterize the different developmental stages present in the cultures. Our results demonstrated that application of Mature-OL media to induce oligodendrocyte maturation resulted in significantly (p<0.05) more oligodendrocytes immunopositive for the myelin marker proteolipid protein (PLP) than OPC or Immature-OL media following 5 days in culture, with >70% cells immunoreactive. This demonstrates that our new is able to generate high percentages of oligodendrocytes at specific stages of development. This model will now be utilized to characterized the effect of extracellular Aβ on oligodendrocyte development in vitro.

Item Details

Item Type:Conference Extract
Keywords:alzheimer's disease, myelin, amyloid, oligodendrocyte
Research Division:Biomedical and Clinical Sciences
Research Group:Neurosciences
Research Field:Neurosciences not elsewhere classified
Objective Division:Health
Objective Group:Specific population health (excl. Indigenous health)
Objective Field:Health related to ageing
UTAS Author:Dwyer, ST (Mr Samuel Dwyer)
UTAS Author:Leung, JYK (Dr Jacqueline Leung)
UTAS Author:Kirkcaldie, M (Dr Matthew Kirkcaldie)
UTAS Author:Vickers, J (Professor James Vickers)
UTAS Author:King, A (Professor Anna King)
ID Code:144005
Year Published:2016
Deposited By:Wicking Dementia Research and Education Centre
Deposited On:2021-04-14
Last Modified:2021-05-06
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