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Recombinant vaccine vector-induced protection of athymic, nude mice from influenza A virus infection. Analysis of protective mechanisms

Citation

Karupiah, G and Ramsay, AJ and Ramshaw, IA and Blanden, RV, Recombinant vaccine vector-induced protection of athymic, nude mice from influenza A virus infection. Analysis of protective mechanisms, Scandinavian Journal of Immunology, 36, (1) pp. 99-105. ISSN 0300-9475 (1992) [Refereed Article]

Copyright Statement

Copyright 1992 John Wiley & Sons, Inc. All rights reserved

DOI: doi:10.1111/j.1365-3083.1992.tb02945.x

Abstract

Athymic, nude mice, which normally succumb to virus infection, can resolve infection with recombinant vaccinia virus (rVV) engineered to express IL-2. We have demonstrated that interferon-gamma (IFN-gamma) produced by natural killer (NK) cells and other immunocytes in response to the virus-encoded interleukin-2 (IL-2) is crucial to recovery. Here, we extend this work to show that nude mice, when primed intravenously with rVV co-expressing both IL-2 and an influenza virus haemagglutinin (HA) gene, are also protected following challenge with a lethal dose of homologous influenza virus. A substantial increase in the number of influenza virus-reactive antibody-secreting cells producing antibody of the IgM isotype, but not of the IgG or IgA isotypes, was found in spleens and lungs of the protected mice. Treatment with monoclonal antibodies to IFN-gamma or to the NK marker, as GM1, at challenge and thereafter, led to their death however, though the specific IgM antibody response was unaffected. These data suggest that both specific antibody and non-specific antiviral reactivity are important elements of the protective response and show that this immunization strategy may be used to protect severely immunocompromised individuals.

Item Details

Item Type:Refereed Article
Keywords:Recombinant vaccinia virus; vaccination; influenza A virus; antibody responses
Research Division:Biomedical and Clinical Sciences
Research Group:Immunology
Research Field:Innate immunity
Objective Division:Health
Objective Group:Clinical health
Objective Field:Prevention of human diseases and conditions
UTAS Author:Karupiah, G (Associate Professor Guna Karupiah)
ID Code:142859
Year Published:1992
Web of Science® Times Cited:13
Deposited By:Medicine
Deposited On:2021-02-12
Last Modified:2021-05-26
Downloads:0

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