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Different roles for CD4+ and CD8+ T lymphocytes and macrophage subsets in the control of a generalized virus infection

Citation

Karupiah, G and Buller, RML and Van Rooijen, N and Duarte, CL and Chen, J, Different roles for CD4+ and CD8+ T lymphocytes and macrophage subsets in the control of a generalized virus infection, Journal of Virology, 70, (12) pp. 8301-8309. ISSN 0022-538X (1996) [Refereed Article]

Copyright Statement

Copyright 1996, American Society for Microbiology

DOI: doi:10.1128/JVI.70.12.8301-8309.1996

Abstract

The importance of T-lymphocyte subsets in the control of poxvirus infections is controversial. To determine the relative contribution of lymphocyte subsets important for recovery from infection with ectromelia virus (EV), a natural murine poxvirus pathogen, C57BL/6 (B6) mice lacking functional CD8+ T cells because of disruption of the beta2-microglobulin gene or lacking functional CD4+ T cells because of disruption of the I-(A)beta gene, acutely depleted of CD8+ or CD4+ T cells with monoclonal antibody, or depleted of macrophage subsets by the macrophage suicide technique were used. Recovery from infection was strictly dependent on the effector functions of CD8+ T cells, in the absence of which 100% mortality resulted. This lymphocyte population had demonstrable antiviral activity early in the infection process even before class I major histocompatibility complex (MHC)-restricted CD8+ cytotoxic T-lymphocyte (CTL) activity was detectable. CD4+ T cells were found to be necessary for the generation of an optimal virus-specific, class I MHC-restricted CD8+ CTL response and contributed to virus clearance not involving cytolytic mechanisms. In both models of CD4+ T-cell deficiency, virus clearance was incomplete and persisted at low levels in most organs and at very high levels in the skin, but the animals did not die. The elimination of macrophage subpopulations impeded virus clearance, impaired the generation of class I MHC-restricted antiviral CTL response, and resulted in 100% mortality. These findings establish an absolute requirement for CD8+ and CD4+ T lymphocytes and macrophage subsets in the elimination of a natural murine poxvirus infection and support the idea that macrophages may be essential accessory cells for the generation of class I MHC-restricted antiviral CTL responses.

Item Details

Item Type:Refereed Article
Keywords:CD4 T cells; CD8 T cells; macrophages; antiviral immunity; Ectromelia virus.
Research Division:Biomedical and Clinical Sciences
Research Group:Immunology
Research Field:Cellular immunology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Prevention of human diseases and conditions
UTAS Author:Karupiah, G (Associate Professor Guna Karupiah)
ID Code:142848
Year Published:1996
Web of Science® Times Cited:144
Deposited By:Medicine
Deposited On:2021-02-12
Last Modified:2021-05-25
Downloads:0

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