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The Interferon-Inducible Chemokines MuMig and Crg-2 Exhibit Antiviral Activity In Vivo

Citation

Mahalingam, S and Farber, JM and Karupiah, G, The Interferon-Inducible Chemokines MuMig and Crg-2 Exhibit Antiviral Activity In Vivo, Journal of Virology, 73, (2) pp. 1479-1491. ISSN 1098-5514 (1999) [Refereed Article]

Copyright Statement

Copyright © 1999, American Society for Microbiology. All Rights Reserved.

DOI: doi:10.1128/JVI.73.2.1479-1491.1999

Abstract

MuMig (murine monokine induced by gamma interferon) and Crg-2 (cytokine responsive gene 2) are two murine chemokines of the CXC family that are induced by the interferons (IFNs): MuMig specifically by IFN-g and Crg-2 by IFN-a, IFN-b, and IFN-g. To investigate the biological roles of these chemokines, recombinant vaccinia viruses (rVVs) encoding either MuMig or Crg-2 were constructed. In vitro, the chemokine-encoding rVVs replicated to similar levels to the control virus. Athymic nude mice inoculated with 105 PFU or less of VV-HA-Mig or VV-HA–Crg-2 resolved the infection successfully whereas mice given a similar dose of the control virus VV-HA-TK died from generalized infection. At higher doses, there was mortality in all groups but death was significantly delayed in mice infected with either chemokine-encoding rVV compared with those infected with the control virus. Virus-encoded MuMig and Crg-2 enhanced the cytolytic activity of NK cells and splenic cellularity by two- to threefold and resulted in significant increases in mononuclear cell infiltration in the livers of mice. Using specific neutralizing or depleting antibodies, we have established that the control of rVV replication in athymic nude mice, as a consequence of virus-expressed MuMig and Crg-2, requires NK cells and IFN-a, IFN-b, and IFN-g.

Item Details

Item Type:Refereed Article
Keywords:IFN-g; chemokines; chemotaxis; antiviral immunity; poxviruses
Research Division:Biomedical and Clinical Sciences
Research Group:Immunology
Research Field:Innate immunity
Objective Division:Health
Objective Group:Clinical health
Objective Field:Prevention of human diseases and conditions
UTAS Author:Karupiah, G (Associate Professor Guna Karupiah)
ID Code:142842
Year Published:1999
Web of Science® Times Cited:92
Deposited By:Medicine
Deposited On:2021-02-12
Last Modified:2021-05-25
Downloads:0

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