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The proteomic response is linked to regional lung volumes in ventilator-induced lung injury


Yen, S and Song, Y and Preissner, M and Bennett, E and Wilson, R and Pavez, M and Dubsky, S and Dargaville, PA and Fouras, A and Zosky, GR, The proteomic response is linked to regional lung volumes in ventilator-induced lung injury, Journal of Applied Physiology, 129, (4) pp. 837-845. ISSN 8750-7587 (2020) [Refereed Article]

Copyright Statement

Copyright 2020 the American Physiological Society

DOI: doi:10.1152/japplphysiol.00097.2020


It is unclear how acid-induced lung injury alters the regional lung volume response to mechanical ventilation (MV) and how this impacts protein expression. Using a mouse model, we investigated the separate and combined effects of acid aspiration and MV on regional lung volumes and how these were associated with the proteome. Adult BALB/c mice were divided into four groups: intratracheal saline, intratracheal acid, saline/MV, or acid/MV. Specific tidal volume (sVt) and specific end-expiratory volume (sEEV) were measured at baseline and after 2 h of ventilation using dynamic high-resolution four-dimensional computed tomography (4DCT) images. Lung tissue was dissected into 10 regions corresponding to the image segmentation for label-free quantitative proteomic analysis. Our data showed that acid aspiration significantly reduced sVt and caused further reductions in sVt and sEEV after 2 h of ventilation. Proteomic analysis revealed 42 dysregulated proteins in both Saline/MV and Acid/MV groups, and 37 differentially expressed proteins in the Acid/MV group. Mapping of the overlapping proteins showed significant enrichment of complement/coagulation cascades (CCC). Analysis of 37 unique proteins in the Acid/MV group identified six additional CCC proteins and seven downregulated proteins involved in the mitochondrial respiratory chain (MRC). Regional MRC protein levels were positively correlated with sEEV, while the CCC protein levels were negatively associated with sVt. Therefore, this study showed that tidal volume was associated with the expression of CCC proteins, while low end-expiratory lung volumes were associated with MRC protein expression, suggesting that tidal stretch and lung collapse activate different injury pathways.

NEW & NOTEWORTHY This study provides novel insights into the regional response to mechanical ventilation in the setting of acid-induced lung injury and highlights the complex interaction between tidal stretch and low-end-expiratory lung volumes; both of which caused altered regulation of different injury pathways.

Item Details

Item Type:Refereed Article
Keywords:acid aspiration, lung imaging, mechanical ventilation, ventilator-induced lung injury proteomics, proteomics
Research Division:Biomedical and Clinical Sciences
Research Group:Cardiovascular medicine and haematology
Research Field:Respiratory diseases
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Yen, S (Mr Seiha Yen)
UTAS Author:Song, Y (Dr Yong Song)
UTAS Author:Bennett, E (Ms Ellen Bennett)
UTAS Author:Wilson, R (Dr Richard Wilson)
UTAS Author:Pavez, M (Miss Macarena Pavez)
UTAS Author:Dargaville, PA (Professor Peter Dargaville)
UTAS Author:Zosky, GR (Professor Graeme Zosky)
ID Code:141639
Year Published:2020
Funding Support:National Health and Medical Research Council (1077905)
Deposited By:Menzies Institute for Medical Research
Deposited On:2020-11-05
Last Modified:2020-12-09

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