eCite Digital Repository

Rapid method to obtain high quality nuclear and chromosome images directly from living cells in the Characeae

Citation

Gargiulo, GM and Gemelli, F and Cambrea, G and Ferraro, GB and Crosca, A, Rapid method to obtain high quality nuclear and chromosome images directly from living cells in the Characeae, Phycological Research, 67, (1) pp. 72-76. ISSN 1440-1835 (2019) [Refereed Article]

Copyright Statement

Copyright 2018 Japanese Society of Phycology

DOI: doi:10.1111/pre.12335

Abstract

This study focused on a method based on the capacity of cationic dyes to stain only the nucleus and chromosomes in cells subjected to either acid or alkaline hydrolysis. The method and the squash were optimized for the Characeae and were described in detail. Nuclei from vegetative shoot apices and antheridial filament cells of unfixed, fixed and herbarium material of Nitella opaca were investigated using the Azure A or Toulidine Blue stains. Comparisons with some other staining methods, used in the previous studies, were also reported. The Azure A/Toulidine Blue method is useful to obtain clear images of chromosome morphology comparable or higher to that obtained with Feulgen or Aceto-Orcein. It requires little time and a less complicated procedure in comparison with other staining methods.

Item Details

Item Type:Refereed Article
Keywords:Azur A, karyotype, Nitella, squash method, Toulidine Blue
Research Division:Biological Sciences
Research Group:Biochemistry and cell biology
Research Field:Analytical biochemistry
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Gemelli, F (Ms Federica Gemelli)
ID Code:139821
Year Published:2019
Web of Science® Times Cited:1
Deposited By:Directorate
Deposited On:2020-07-07
Last Modified:2020-08-17
Downloads:0

Repository Staff Only: item control page